Human BRAF Gene V600E Mutation Detection Kit

Product Features:

• High Sensitivity:  accurately detect 1% of mutantDNA from 10ng of the wild-type gene
• High Specificity:  effectively improve the testing specificity by ARMS-PCRdetection technology
• High Accuracy: closed tube reaction effectively prevent false negative and false positive results through the standard internal system and UNG enzyme system
• Quick & Easy: easy to operate with reliable results analysis, 90 mins to complete

Product Information

Clinical Significance

BRAF, ARAF, CRAF gene belong to the RAF gene family and encodes the serine-threonine protein kinase in the MAPK pathway, which transduce signals from RAS to MEK1/2, thereby participating in the regulation of multiple biological events in cells. BRAF mutations occur in nearly 8% of human tumors, mainly in colon cancer, melanoma and papillary thyroid cancer.

According to statistics,about 15% of colon cancer patients have somaticBRAF gene mutations, and 11% of the mutations are located in the glycine ring of exon 11; 89% of the mutations occurred in the activation region of exon 15, in which about 92% were located at nucleotide1799 (T mutation is A), resulting in the substitution of its coded valine by glutamic acid (V600E). Studies have shown that anti-EGFR targeted therapy is ineffective for colon cancer patients with wild-type KRAS gene and BRAF V600E mutation.

In the case of wild-type KRAS patients, the overall survival period and non-progressive survival period of BRAF mutant patients treated with cetuximab and paniximab , were significantly lower than those wild-type patients.

BRAF Gene Corresponding Targeted Drugs